宿主:小鼠
用途:WB,ICC, IHC
交叉反应性:人,小鼠,大鼠
分子量:43KDa
推荐稀释比例:WB(1:1000),IF(1:20),IHC(1:20),ICC(1:20)
克隆性:单克隆抗体
About this Antibody | |
Name | β-Actin Mouse Monoclonal Antibody |
Category | Monoclonal antibody (mAb); Primary antibody |
Isotype | IgG1 |
Purification | Affinity chromatography |
About the Immunogen | |
Immunogen | synthetic peptide (KLH-coupled) |
Gene ID | 60/11461/81822 |
SwissProt | P60709/P60710/P60711 |
Synonyms | BRWS1; PS1TP5BP1 |
Category | Cytoskeletal Signaling |
Background | Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β-Actin is generally used as an internal control for experimental error. |
注意事项:
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
2. 回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
储存条件:-20℃,有效期一年。
相关搜索:小鼠抗β-Actin抗体,β-Actin Mouse Monoclonal Antibody